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KMID : 1094720090140030338
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Biotechnology and Bioprocess Engineering
2009 Volume.14 No. 3 p.338 ~ p.344
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Comparative Study of Digestive Enzymes of Squid (Todarodes pacificus) Viscera after Supercritical Carbon Dioxide and Organic Solvent Extraction
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Uddin Salim
Ahn Hyang-Min
Hideki Kishimura
Chun Byung-soo
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Abstract
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Three major classes of digestive enzymes of squid viscera were characterized following extraction of oil by supercritical carbon dioxide (SCO2) and organic solvent, n-hexane. Squid viscera were extracted at temperature, 35~45°C and pressure, 15~25 MPa for 2.5 h by SCO2 with a constant flow rate of 22 g/min. Oil extraction yield increased with the increasing of extraction pressure and temperature. The highest oil extracted residues of squid viscera were used for characterization of digestive enzymes. The activities of protease, lipase, and amylase were highest in n-hexane treated squid viscera samples and lowest in SCO2 treated samples. The crude extracts of SCO2 and n-hexane treated squid viscera samples showed almost same optimum pH and pH stability for each of the digestive enzymes. The optimum temperature of protease, lipase, and amylase were found to almost similar in SCO2 and n-hexane treated samples. But the thermal stability for each digestive enzyme in SCO2 treated squid viscera were slightly higher than that of n-hexane treated squid viscera. Studies using SDS-PAGE showed no significant differences in protein patterns of the crude extracts of untreated and SCO2 and n-hexane treated squid viscera indicating no denaturation of proteins.
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KEYWORD
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digestive enzymes, squid viscera, supercritical carbon dioxide, n-hexane, enzyme activity
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